Vaginal and Uterine Microbiomes during Puerperium in Dairy Cows

Knowledge of how vaginal and uterine microbiomes are composed is essential to prevent diseases understand their pathogenesis. For this purpose, 50 dairy cows were involved in a prospective trial. Cows clinically examined swabs taken (day 2, 4, 6 14 p.p.), followed up by clinical examination on day 21 ± 1 41 1. All samples (n = 364) analyzed with cultural bacteriological methods bacteria identified MALDI-TOF MS. In animals pathological puerperium, bacterial diversity was reduced the genera Fusobacteria spp., Bacteroides spp. Helcococcus occurred significantly more frequently. By contrast, other well-known pathogens such as Escherichia Trueperella pyogenes did not show significant differences between healthy diseased cattle. Bacterial occurrences vagina uterus sometimes correlated. that suffered from puerperal disorders showed lower incidence coagulase- negative staphylococci even before symptoms occurred. This may point towards protective role microbiome. addition, we discuss several shortcomings calving hygiene might have contributed high percentage study farm (58.7%).